JPKはDresden & Cambridge UniversitiesのDr Jochen Guckのグループの研究をご紹介します

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Dr Jochen Guck’s PhD student in Cambridge, Kathrin Holtzmann, working with JPK's NanoWizard® AFM.

JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports on the multi-faceted research projects of Dr Jochen Guck who runs simultaneous research at theUniversities of Dresden and Cambridge.

The biophysicist Dr. Jochen Guck has recently started his Humboldt Professorship of Cellular Machines at the Biotechnology Center of the TU Dresden (BIOTEC) while continuing to lead a team at the Cavendish Laboratory at Cambridge University (UK). His work at BIOTEC focuses on the development of new biophysical approaches for stem cell research, blood cell diagnostics and neuroregeneration. One of his main tools for characterization of biomaterials are the NanoWizard® systems from JPK Instruments. Unlike many other users, he does not apply them for basic scanning measurements but uses them to study cellular behaviour and to quantify their interactive forces.

Talking about his research, Professor Guck said “Most of our work uses AFM for mapping the mechanical properties of tissues. It is starting to be recognized that cells respond to the mechanical properties of the environmentthey are in contact with. They can differentiate into different lineages when incontact with stiff or compliant surfaces. Or they migrate towards stiffer areasor softer areas - a phenomenon called durotaxis. We are one of the few groups that have started to consider this mechanosensitivity of cells in the centralnervous system (CNS); in the context of neural development and pathological disorders. One important prerequisite for these studies is to know quantitatively, how stiff or how soft CNS tissues actually are so that we can mimic this environment in vitro, and whether there are heterogeneities that cells could respond to. If everything is the same, there is no cue for migration for example. This is where we have pioneered the use of AFMs for the mapping of mechanical properties of CNS tissues with high spatial resolution.”

Continuing about his experiences in working with JPK, he said “NanoWizard is perfect for measuring mechanical properties of biological cells and tissues,while keeping the cells and tissues in their physiological environment at the right temperature and their preferred medium (BioCell™). And, we can correlate mechanical mapping with optical microscopy such as bright-field or fluorescence microscopy. This helps us to know what we are actually measuring: which cell type, which state (quiescent or activated) or which area in a tissue.”