オーストラリア、モナシュ大学における NanoWizard® AFM システムを使用した フォース測定についてご報告いたします。
JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports on the use of the NanoWizard® AFM system in the School of Chemistry at Monash University in Australia.
Dr Rico Tabor leads the Soft Materials and Colloids Group in the School of Chemistry at Monash University. Research focuses on several areas. At a fundamental level, the group is interested in exploring the interactions that cause aggregation and assembly, in everything from nanoparticles, droplets and graphene to proteins and cells. This ranges from quite fundamental studies of things like the van der Waals force (of quantum origin) and electrical double-layer interactions, right through to unwinding protein molecules and the forces experienced by emulsion droplets armoured with 2D nanomaterials. Inparticular, they are exploring ways in which such forces can be exploited, such as whether changing something as simple as pH can cause particles or drops to coalesce or stick together. In other circumstances, more 'advanced' stimuli such as light or magnetic fields are used to induce the chemical or physical effects that are required.
Dr Tabor describes some of the techniques that are used: "We use many tools frequently from simple things like surface tension all the way up to large-scale facilities such as the Australian Synchrotron and the neutron facilities at the Bragg Institute. However, AFM is the only technique that can tell us directly about forces in such a wide range of systems. And this information is central in understanding why things are stable, or why they phase separate. If we can understand the forces between our dispersed items, be they particles, drops orgraphene sheets, then we can design materials that have the characteristics that we want. Although there are other force measuring techniques, for the family of materials that we are interested in - drops, particles, bubbles, etc., AFM is the gold standard in terms of applicability, versatility and precision. With AFM, you can explore so much more: deformation, mechanics, interactions, etc. and in almost any system. Some things such as biological molecules, surfactant assemblies and other soft, labile materials are almost impossible to image in electron microscopy, but with judicious cantilever choice, the AFM often comes to the rescue!"
So why did Dr Tabor choose the NanoWizard® AFM from JPK? "I have used several commercial AFMs but, for me, JPK AFMs appealed because of the durability when working in liquids - most of our systems are wet at one stage or another. We can just drop our sample into a Petri dish and off we go! The hassle of the fiddly liquid cells found with other AFMs used to frustrate me no end. The versatility of the JPK is a great bonus, and the setup time is so fast - I can be ready and imaging within 5 minutes, which I think beats any of the other comparable models. Combine this with automated force curve acquisition and analysis, and you have a powerful system for force measurement."